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KMID : 0848120210460010023
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International Journal of Oral Biology
2021 Volume.46 No. 1 p.23 ~ p.29
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Apoptotic activity of demethoxycurcumin in MG-63 human osteosarcoma cells
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Kang Kyeong-Rok
Kim Jae-Sung
Kim Tae-Hyeon
Seo Jeong-Yeon
Park Jong-Hyun
Chun Hong-Sung
Yu Sun-Kyoung
Kim Heung-Joong
Kim Chun-Sung
Kim Do-Kyung
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Abstract
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Demethoxycurcumin (DMC), which is a curcuminoid found in turmeric, has anti-proliferative effects on cancer cells. However, the effect of DMC on osteosarcoma has not been established. The aim of this study was to examine the effects of DMC on cell growth and apoptosis induction in MG-63 human osteosarcoma cells. This study was investigated using 3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromid assay, Live/Dead cell assay, 4’, 6-diamidino-2-phenylindole staining, and immunoblotting in MG-63 cells. DMC induced MG-63 cell death in a dosedependent manner, with an estimated IC50 value of 54.4 μM. DMC treatment resulted in nuclear condensation in MG-63 cells. DMC-induced apoptosis in MG-63 cells was mediated by the expression of Fas and activation of caspase-8, caspase-3, and poly (ADP-ribose) polymerase. Immunoblotting results showed that Bcl-2 and Bcl-xL were downregulated, while Bax and Bad were upregulated by DMC in MG-63 cells. These results indicated that DMC inhibits cell proliferation and induces apoptotic cell death in MG-63 human osteosarcoma cells via the death receptormediated extrinsic apoptotic pathway and mitochondria-mediated intrinsic apoptotic pathway.
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KEYWORD
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Demethoxycurcumin, Cell death, Apoptosis, Anticancer effect
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